PrPTSE detection

Nanoparticule Prion Capture

Magnetic Nanoparticle Capture of Prion Infectivity

2010: 1 year / Grant: 34 000 euros

Project: The “Nanoparticle prion capture” project proposed to evaluate the efficacy of a method
combining the specific capture of PrPTSE with magnetic nanoparticles and the PMCA detection
technique. As magnetic nanoparticles were shown bythe investigatorsto attach to prions, they also
proposed to assess whether theycould, in addition to concentrating prion and facilitating its detection
following PMCA, remove prion infectivity from infected samples.

Main results and related published data: Superparamagnetic nanoparticles were shown to specifically capture PrPTSE in brain homogenates from mice infected with numerous prion strains (murine RML, Me7, the BSE adapted stain 301C and hamster brain infected with Sc237 and 139H).
This particularcapture of prions wasshown to be compatible with the PMCA detection method since sequestrated prions were able to constitute seed for their amplification with the PMCA method. In addition to an improvement in detection, the magnetic nanoparticles have the capacity to remove prion infectivityfrom infected samples, therefore suggesting their usefulnessin biologically derived products.

Superparamagnetic nanoparticle capture of prions for amplification. Miller MB1, Supattapone S. J Virol. 2011 Mar;85(6):2813-7. doi: 10.1128/JVI.02451-10. Epub 2011 Jan 12.