PrPTSE detection

ExoQuiC

An exosome strategy for the diagnosis of human prion diseases

2013: 19 months / Grant: 35 000 euros

Project : Based on promising studies showing the possibility to detect PrPTSE (by western blotting and ELISA) in the exosomal fractions of plasma from prion infected hamsters, the initial objective of the project “ExoQuiC” wasto determine whether the use of the QuIC method on exosomal fractions could improve the detection of PrPTSE even during the presymptomatic phase of the disease. The ultimate aim was to adapt this method to human plasma and CSF samples.

Main results and related published data: Improvements of exosomal purification fractions, as well as precipitation with thyroglobulin, have been obtained on plasma samplesfrom prion infected hamsters and scrapie infected sheep. These steps were necessary to remove plasma components, such as immunoglobuline, that could interfere with PMCA method or PrPTSE detection by immunochemistry. An important result, that remainsto be refined, wasthat 20% of PrPTSE infectivity seemed to be associated with the exosomal fraction.