Fundamental research Experimental models

TSETC

Experimental treatment with RAP of TSE infection in cell cultures and sCJD infection in HuTg mice.

2008: 1 year / Grant: 40 000 euros

Project: As prion contamination was matter ofconcernsthe field ofcell therapy using bone-marrow derived Mesenchymal stem cells (MSC). The aim of the TSETC project address this risk by checking the susceptibility Mesenchymal stem cells from bone marrows to human prions.

Main results and related published data: A first result was the demonstration that MSC cells could be infected with mouse adapted GSS strain (Fukuoka1) and too the vCJD mouse adapted strain.
An increase of the level of PrPC was observed during the imortalisation phase for generating MSC in culture. Following prion infection, the cells were forced to underwent a spontaneous transformation. The MSC Sca-1 stem cell antigen was also present in the majority of the cells. In addition, More recently and consequentlyto this work, MSC cells derived from bone marrow of prion infected mice were shown to be devoided of PrP expression as well as PrPTSE. When maintained in culture the cells began an immortalization process. However, depending on the culture condition cells could maintained persistent replication or not.