Fundamental research Experimental models

CGSS

Infectivity titrations of blood components from chimpanzees infected with the agent of Gerstmann-Sträussler-Scheinker Syndrome in mice and comparison of sensitivity of mouse bioassays

2010: 2 years / Grant: 11 000 euros

Project: The aim of the project“CGSS” wasto establish valuable toolsthatcould be used to develop ante-mortem teststo detect prion infectivity. Because of the risk ofsecondarytransmission by blood transfusion, such tools are crucialy needed. However human CJD infected blood samples necessary to set up those tests are rare. The project proposed to use and check whether blood from chimpanzee infected with GSS or from macaques experimentally infected with vCJD could be good candidates to replace human samples. The sensitivity of several TSE mouse models for prion titration was also proposed to be compared in this project.

Main results and related published data: To generate blood reference materials, macaques have been infected. Plasma collected at the terminal phase of the disease were analysed for PrPTSE by PMCA. PrPTSE was detected in plasma from infected monkeys following PMCA. They also showed that mouse models C57BL/6 and RIII J/S wild-type mice were more sensitive to brain samples froms macaques infected with the vCJD agent than to vCJD agent from human itself.