Fundamental research Experimental models

Blood Animal Model

An assessment of the relevancy of sheep and mice animal model for blood safety investigation

2009: 1 year / Grant: 30 000 euros

Project: This project was complementary to the “Endogenous Blood Infectivity Tool” project from O. Andreoletti and proposed to determine whether and which TSE animal models were relevant for studying blood derivatives safety and risk assessments.

Main results and related published data: The biological relevance of measuring the infectious titers from blood componenent in animal models was studied by comparing the occurrence of aTSE following the transfusion of blood derivatives in sheep or intra-cerebral inoculation in Tg338 mice overexpressing the ovine PrP. Greater virulence was observed when 200µl of blood from asymptomatic infected sheep was transfused in sheep than whith 200ml of Prion spiked blood containing 10³ID of prion infectivity or when intracerebrallyinoculated in tg338 mice. More recently and consecutively to this work, two transgenic mouse models Tg340Hu (overexpressing the human PrP) and Tg110Bov (overexpressing the bovine PrP), known for their high sensitivity to the vCJD or sCJD TSE agents, were used to evaluate the infectious titer in blood fractions from vCJD and sCJD patients.